of pharmaceutical compounds from plant cell cultures
The participant will be engaged in research to investigate a hypothesis and will learn and apply knowledge and skills to the production of valuable pharmaceuticals from cell cultures. They will learn 1) techniques for growing and studying plant cell cultures and 2) chemical analysis methods for isolating, quantifying, and identifying compounds produced by the cell cultures. In the process of participating in our research, the participant will read the literature, formulate a hypothesis, design experiments to test the hypothesis, plan and perform the experiments, and analyze and interpret the results.
will be expected to engage in all aspects of the research, work closely
with a graduate student mentor, and make the most of this learning opportunity!
They will keep a laboratory notebook detailing their experimental methods
and findings. They will conduct a growth and production curve of the cell
line being studied; this will include preparing the culture media, inoculating
the flasks with cells, taking samples of cells every three days, preparing
and performing the chemical analysis. They will write up their results
in a report form under the guidance of their mentors and also have the
opportunity to present their results to the research group.
motivated, focused, hard-working, a desire to learn / be challenged, conscientious,
Lab safety/issues unique to this laboratory
Chemicals and organic solvents are used; special care in handling compounds which have medicinal activity.
www.coe.neu.edu/~clee; Joel L. Swerdlow, Nature's Rx, National Geographic Magazine, April 2000
Download MS Word version of this assignment.
Click here for RET 2005 research presentation.